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pe anti human cd80  (4A Biotech)

 
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    4A Biotech pe anti human cd80
    Pe Anti Human Cd80, supplied by 4A Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+human+cd80/anti+cd80+human+pe/pmc12894399-100-27-30
    Average 86 stars, based on 1 article reviews
    pe anti human cd80 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Mechanisms and safety evaluation of stir-fried atractylodis macrocephalae rhizoma with aurantii fructus in the treatment of inflammatory bowel disease: a study based on organ-on-a-chip model
    Article Snippet: .. The differentiated THP-1 cells were detached with trypsin-EDTA and collected by centrifugation at 1,000 rpm for 5 min. For cell surface antigen staining, macrophages were stained with PE anti-Human CD80 (4A Biotech, China, FHN80-01-100) at 4 °C for 30 min. After washing, the cell fixation solution was added and the samples were incubated at room temperature in the dark for 10 min. .. Subsequently, a membrane-permeabilizing agent was added to permeabilize the cell membranes for 15 min. For intracellular antigen staining, macrophages were stained with PE/Cy7 anti-Human CD206 (4A Biotech, China, FHN206-01-100) at room temperature for 30 min.

    Staining:

    Article Title: Mechanisms and safety evaluation of stir-fried atractylodis macrocephalae rhizoma with aurantii fructus in the treatment of inflammatory bowel disease: a study based on organ-on-a-chip model
    Article Snippet: .. The differentiated THP-1 cells were detached with trypsin-EDTA and collected by centrifugation at 1,000 rpm for 5 min. For cell surface antigen staining, macrophages were stained with PE anti-Human CD80 (4A Biotech, China, FHN80-01-100) at 4 °C for 30 min. After washing, the cell fixation solution was added and the samples were incubated at room temperature in the dark for 10 min. .. Subsequently, a membrane-permeabilizing agent was added to permeabilize the cell membranes for 15 min. For intracellular antigen staining, macrophages were stained with PE/Cy7 anti-Human CD206 (4A Biotech, China, FHN206-01-100) at room temperature for 30 min.

    Incubation:

    Article Title: Mechanisms and safety evaluation of stir-fried atractylodis macrocephalae rhizoma with aurantii fructus in the treatment of inflammatory bowel disease: a study based on organ-on-a-chip model
    Article Snippet: .. The differentiated THP-1 cells were detached with trypsin-EDTA and collected by centrifugation at 1,000 rpm for 5 min. For cell surface antigen staining, macrophages were stained with PE anti-Human CD80 (4A Biotech, China, FHN80-01-100) at 4 °C for 30 min. After washing, the cell fixation solution was added and the samples were incubated at room temperature in the dark for 10 min. .. Subsequently, a membrane-permeabilizing agent was added to permeabilize the cell membranes for 15 min. For intracellular antigen staining, macrophages were stained with PE/Cy7 anti-Human CD206 (4A Biotech, China, FHN206-01-100) at room temperature for 30 min.



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    Image Search Results


    Human M1-polarized macrophages were exposed to A075G3 and A075W3 for 48 h. Positivity for CD80, a pro-inflammatory surface marker, was assessed by flow cytometry and expressed as mean fluorescence intensity (MFI) ± SD ( n = 4 for CTR, A075G3 and A075W3). * p < 0.05 (A075W3 vs CTR).

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Methods and Technical Issues for Optimizing the Production of Hydrogels Containing Decellularized Wharton’s Jelly

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    Figure Lengend Snippet: Human M1-polarized macrophages were exposed to A075G3 and A075W3 for 48 h. Positivity for CD80, a pro-inflammatory surface marker, was assessed by flow cytometry and expressed as mean fluorescence intensity (MFI) ± SD ( n = 4 for CTR, A075G3 and A075W3). * p < 0.05 (A075W3 vs CTR).

    Article Snippet: PE conjugated antihuman CD80 Antibody (clone REA661) from Miltenyi Biotec (Germany).

    Techniques: Marker, Flow Cytometry, Fluorescence

    Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, CD80 and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.

    Journal: Vaccines

    Article Title: Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer Immunotherapy

    doi: 10.3390/vaccines14040329

    Figure Lengend Snippet: Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, CD80 and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.

    Article Snippet: ACK Lysis Buffer (E-CK-A105), mouse bone marrow-derived dendritic cells (BMDC) induction and identification kit (XJM003), including antibodies of Elab Fluor 488-CD11c, PE/Cyanine7-CD80, PE-CD86, APC-CD40, were purchased from Elabscience Biotechnology (Wuhan, China).

    Techniques: Staining, Laser-Scanning Microscopy, Flow Cytometry, Expressing